Friday, November 13 | 9:15–10:15 am
POSTER ST152
Enhancing Tumor ROI Enrichment from FFPE Lung Cancer Tissue Using the QCPRECISE™ Workflow
Presenter: Vidyodhaya Sundaram, PhD
Authors: V. Sundaram, J. Butler, B. Chaudhuri, S. Karudapuram, K. Konigsfeld, D. Mahesh, N. Sambasivam Durairaju
ABSTRACT
Accurate enrichment of tumor-rich regions from FFPE tissue is critical for downstream molecular analysis, yet manual macrodissection is labor-intensive and can vary in tissue selection and recovery. This pilot study evaluated the QuantumCyte QCPRECISE™ precision tissue enrichment platform against conventional manual macrodissection for DNA extraction from FFPE lung cancer sections. Pathologist-annotated regions of interest on H&E slides guided enrichment of stained and unstained sections. DNA was purified with the BioChain xTractPure FFPE DNA Extraction Kit, assessed for concentration and integrity on the Agilent TapeStation, and analyzed with Tsunami™ EGFR and KRAS mutation-enrichment assays for orthogonal validation.
