Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Nucleus Cyto-Tracer, pCT-H2B-GFP (CMV) fuses an H2B tag to copGFP, resulting in copGFP-labeling of the nucleus. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080.
  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Label the nucleus in a range of cell types

Choose the Cyto-Tracer that’s right for you


Promoter (Cat.#) Cyto-Tracer Target Location Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFP Plasma Membrane Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFP Inner Leaflet of Membrane Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFP Mitochondria Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1) pCT-ER-GFP Endoplasmic Reticulum ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1) pCT-Golgi-GFP Golgi Apparatus Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1) pCT-Secretory-GFP ER-Golgi  Vesicles Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1) pCT-Lyso-GFP Lysosome Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1) pCT-Endo-GFP Endosome RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1) pCT-Endo-RFP Endosome RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFP Peroxisome Peroxisomal consensus signal
CMV (CYTO109-PA-1/CYTO109-VA-1) pCT-Autophago-GFP
Autophagosome LC3
CMV (CYTO109R-PA-1/CYTO109R-VA-1) pCT-Autophago-RFP Autophagosome LC3
CMV (CYTO120-PA-1/CYTO120-VA-1) pCT-CD63-GFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO120R-PA-1/CYTO120R-VA-1) pCT-CD63-RFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1) pCT-CD9-GFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1) pCT-CD9-RFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1) pCT-CD81-GFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1) pCT-CD81-RFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFP Cytoskeleton β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFP Cytoskeleton α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1) pCT-Tublin-RFP Cytoskeleton α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1) pCT-MAP4-GFP Microtubule Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFP Nucleus H2B
CMV (CYTO114-PA-1/CYTO114-VA-1) pCT-Apoptosis-Luc  Caspase 3/7 activation Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1) pCT-GFP-BAX Cytosol to Mitochondria BAX
CMV (CYTO116-PA-1/CYTO116-VA-1) pCT- Catenin-GFP Cytosol to Nucleus/Cell Membrane β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1) pCT-CMV-PSD95-GFP Dendrite Membranes PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFP Cytosolic Compartment GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1) pCT-Cyto-RFP Cytosolic Compartment RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1) pCT-Cyto-GFpLuc GFP and Luciferase Control GFP and Luciferase (Untagged)

Supporting Data

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location


Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action