RA820A-1

Overview

Get your exosomal RNA profiling studies off the ground fast 

With everything you need for profiling exosomal RNAs from serum, plasma, or ascites fluid, SBI’s Human Complete SeraMir Exosome RNA Amplification & Profiling Kit is a great way to get started with exoRNA profiling. The kit includes the fast and efficient ExoQuick Exosome Isolation Reagent, phenol-free SeraMir exosome RNA purification reagents and columns, reagents for cDNA synthesis and amplification, and one 384-well plate with human miRNAs and control wells for quantitation of isolated exoRNAs. (NOTE: for exoRNA isolation from other biofluids, try the Human Complete SeraMir Kit for Media and Urine).
  • Reliable, reproducible exoRNA isolation from serum, plasma, or ascites fluid
  • Sensitive detection via exoRNA amplification
  • Everything you need including 384-well plate of human miRNAs for qPCR profiling
Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

Choose the SeraMir Kit that’s right for you

Cat. # Name Kit includes
ExoQuick or ExoQuick-TC SeraMir RNA columns and reagents SeraMir cDNA synthesis and amplification reagents 384-well plate miRNAs for human, mouse, or rat
RA800A-1 Complete SeraMir Exosome RNA Amplification Kit ✔ ✔ ✔  
RA800TC-1 Complete SeraMir Exosome RNA Amplification Kit for Media and Urine ✔ ✔ ✔  
RA806A-1 SeraMir Exosome RNA Purification Kit ✔ ✔    
RA806TC-1 SeraMir Exosome RNA Purification Kit for Media and Urine ✔ ✔    
RA808A-1 SeraMir Exosome RNA Purification Column Kit   ✔    
RA820A-1 Human Complete SeraMir Exosome RNA Amplification and Profiling Kit ✔ ✔ ✔ ✔
RA820TC-1 Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine ✔ ✔ ✔ ✔
RA821A-1 Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit ✔ ✔ ✔ ✔
RA821TC-1 Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine ✔ ✔ ✔ ✔
RA822A-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit ✔ ✔ ✔ ✔
RA822TC-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine ✔ ✔ ✔ ✔

How It Works

Quickly and easily profile exoRNAs

  • Isolate exosomes from patient biofluids with the included ExoQuick reagent
  • Purify exoRNAs with SeraMir columns
  • Measure expression levels of 380 human miRNAs in a 384-well qPCR assay

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

 

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Three validated reference controls included on the SeraMir Profiling qPCR plate

Human miRNA profiling qPCR plate includes three validated reference controls

 

Excellent technical replicates across an 8-log range

Excellent technical replicates across 8 logs

 

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.